Testicular 25-hydroxycholesterol: An alternate substrate for steroidogenesis in reptiles.
Gen Comp Endocrinol
; 314: 113906, 2021 12 01.
Article
em En
| MEDLINE
| ID: mdl-34534545
ABSTRACT
The current study in wall lizards Hemidactylus flaviviridis was designed to ascertain that Leydig cells utilize testicular macrophage-derived 25-hydroxycholesterol (25-HC) for steroidogenesis. Leydig cells (LC) collected from regressed testes when incubated with 25-HC that was obtained from HPLC-eluted fraction of testicular macrophage-conditioned medium (TMCM), lyophilized and reconstituted in culture medium (0.5 µg/ml/well), produced considerably higher amount of testosterone. A similar observation was made when Leydig cells were incubated with varying concentrations of commercial 25-HC. Testosterone production by LC increased in a concentration-dependent manner. Taken together, it is evident that LC utilize 25-HC as a substrate for testosterone biosynthesis. To examine the gonadotropic regulation of steroid biosynthesis utilizing 25-HC as substrate, ovine follicle-stimulating hormone (FSH) that regulates both the testicular functions in lizards was used. Leydig cells were incubated with combinations of FSH and 25-HC as follows 0 h FSH + 12 h 25-HC, 0 h 25-HC + 12 h FSH. As compared to respective controls, a marked increase in testosterone production was observed in response to FSH indicating that gonadotropin up-regulates uptake of 25-HC as a substrate for testosterone biosynthesis.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Testículo
/
Testosterona
/
Hidroxicolesteróis
/
Células Intersticiais do Testículo
/
Lagartos
Limite:
Animals
Idioma:
En
Revista:
Gen Comp Endocrinol
Ano de publicação:
2021
Tipo de documento:
Article
País de afiliação:
Índia