Alteration in meibum lipid composition and subjective symptoms due to aging and meibomian gland dysfunction.
Ocul Surf
; 26: 310-317, 2022 10.
Article
em En
| MEDLINE
| ID: mdl-34666148
ABSTRACT
PURPOSE:
To investigate the alteration in lipid composition of meibum, objective clinical signs, and subjective symptoms associated with aging and meibomian gland (MG) dysfunction (MGD).METHODS:
In 10 MGD patients [4 males/6 females, mean age 65.6 ± 7.9 years (range 50-79 years)] and 24 healthy volunteer subjects [youngsubjects:
6 males/6 females, mean age 25.7 ± 3.8 years (range 20-35 years), elderlysubjects:
6 males/6 females, mean age 58.4 ± 7.5 years (range 50-79 years)], three objective clinical signs were evaluated MG orifice obstruction, meibum score, and tear film lipid layer interference pattern. Subjective symptoms were analyzed via a 15-item questionnaire. After careful collection of meibum samples, comprehensive lipid analysis was performed via liquid chromatography-mass spectrometry. Data was analyzed via JMP® ver. 13 (SAS Institute, Inc., Cary, NC) statistical analysis software.RESULTS:
In the MGD patients and elderly subjects, there was a significant decrease in non-polar lipids such as cholesterol esters (ChEs), while a significant increase in polar lipids [cholesterol (Ch), (O-acyl)-ω-hydroxy fatty acid (OAHFA), and free fatty acid (FA)] in total lipids (Tukey-Kramer test p < 0.05). Triglyceride was significantly increased only in MGD patients (p < 0.05). Symptom scores representative of vision quality (i.e., blurred vision/haziness) were significantly negatively-correlated with the ratio of the non-polar lipid ChE, while significantly positively correlated with the polar lipids Ch, OAHFA, and FA (Spearman's rank correlation coefficient p < 0.05).CONCLUSIONS:
Our findings revealed that both MGD and aging affect the composition ratio of major meibum lipids, resulting in the appearance of subjective symptoms.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Disfunção da Glândula Tarsal
Tipo de estudo:
Diagnostic_studies
Limite:
Adult
/
Aged
/
Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Ocul Surf
Ano de publicação:
2022
Tipo de documento:
Article