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miR-378-3p alleviates contusion spinal cord injury by negatively regulating ATG12.
Zhang, Haocong; Yu, Hailong; Yang, Huifeng; Zhan, Yang; Liu, Xinwei.
Afiliação
  • Zhang H; Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, China.
  • Yu H; Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, China.
  • Yang H; Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, China.
  • Zhan Y; Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, China.
  • Liu X; Department of Orthopaedics, General Hospital of Northern Theater Command, Shenyang, China.
Int J Exp Pathol ; 102(4-5): 200-208, 2021 08.
Article em En | MEDLINE | ID: mdl-34709686
ABSTRACT
MicroRNAs (miRNAs or miRs) serve essential roles in the pathogenic process of spinal cord injury (SCI). The present study investigated the role of miR-378-3p and autophagy-related 12 (ATG12) in SCI. RT-qPCR was used to detect the mRNA expression levels of miR-378-3p and ATG12. Cell viability and membrane integrity were evaluated using CCK-8 and LDH assays. For the analysis of the interaction between miR-378-3p and ATG12, a dual-luciferase reporter assay was conducted. The hindlimb function of rats was detected with the Basso, Beattie and Bresnahan score, and the motor deficit index score was used to evaluate nerve function. Using these approaches, it was identified that miR-378-3p expression was downregulated, while that of ATG12 was upregulated in SCI tissues and in cells exposed to hypoxia. Hypoxia repressed the expression of miR-378-3p via hypoxia-inducible factor 1-α. The overexpression of miR-378-3p exerted anti-apoptotic effects on nerve cells by directly repressing ATG12. The infusion of miR-378-3p improved hindlimb motor function and the neurological functions of rats with contusion SCI, which contributed to amelioration of functional deficits and the relief of contusion SCI. Therefore, it was concluded that upregulated expression of miR-378-3p in PC12 or N2A cells repressed the apoptosis of nerve cells, and the administration of miR-378-3p in model rats with contusion SCI improved neurological and motor functions.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / MicroRNAs / Proteína 7 Relacionada à Autofagia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Traumatismos da Medula Espinal / MicroRNAs / Proteína 7 Relacionada à Autofagia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Int J Exp Pathol Assunto da revista: PATOLOGIA Ano de publicação: 2021 Tipo de documento: Article País de afiliação: China