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Multiple Immunostainings with Different Epitope Retrievals-The FOLGAS Protocol.
von Schoenfeld, Anna; Bronsert, Peter; Poc, Michael; Fuller, Andrew; Filby, Andrew; Kraft, Stefan; Kurowski, Konrad; Sörensen, Kristin; Huber, Julia; Pfeiffer, Jens; Proietti, Michele; Stehl, Verena; Werner, Martin; Seidl, Maximilian.
Afiliação
  • von Schoenfeld A; Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Bronsert P; Clinic for Internal Medicine, Berlin Jewish Hospital, 13347 Berlin, Germany.
  • Poc M; Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Fuller A; Tumorbank Comprehensive Cancer Center Freiburg, University of Freiburg, 79106 Freiburg, Germany.
  • Filby A; Core Facility for Histopathology and Digital Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Kraft S; Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Kurowski K; Klinik Gut St. Moritz, 7500 St. Moritz, Switzerland.
  • Sörensen K; Flow Cytometry Core Facility, Innovation, Methodology and Application Research Theme, Leech Building, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
  • Huber J; Flow Cytometry Core Facility, Innovation, Methodology and Application Research Theme, Leech Building, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne NE1 7RU, UK.
  • Pfeiffer J; Center for Dermatopathology, 79106 Freiburg, Germany.
  • Proietti M; Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Stehl V; Tumorbank Comprehensive Cancer Center Freiburg, University of Freiburg, 79106 Freiburg, Germany.
  • Werner M; Core Facility for Histopathology and Digital Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
  • Seidl M; Institute for Surgical Pathology, Medical Center-University of Freiburg and Faculty of Medicine, University of Freiburg, 79106 Freiburg, Germany.
Int J Mol Sci ; 23(1)2021 Dec 25.
Article em En | MEDLINE | ID: mdl-35008649
ABSTRACT
We describe a sequential multistaining protocol for immunohistochemistry, immunofluorescence and CyTOF imaging for formalin-fixed, paraffin-embedded specimens (FFPE) in the formalin gas-phase (FOLGAS), enabling sequential multistaining, independent from the primary and secondary antibodies and retrieval. Histomorphologic details are preserved, and crossreactivity and loss of signal intensity are not detectable. Combined with a DAB-based hydrophobic masking of metal-labeled primary antibodies, FOLGAS allows the extended use of CyTOF imaging in FFPE sections.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Imunofluorescência / Inclusão em Parafina / Formaldeído / Epitopos Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Alemanha
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Coloração e Rotulagem / Imunofluorescência / Inclusão em Parafina / Formaldeído / Epitopos Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2021 Tipo de documento: Article País de afiliação: Alemanha