A novel signal amplification biosensor for detection of Cd2+ based on asymmetric PCR.
Spectrochim Acta A Mol Biomol Spectrosc
; 271: 120885, 2022 Apr 15.
Article
em En
| MEDLINE
| ID: mdl-35051799
ABSTRACT
In this work, a novel signal amplification biosensor was utilized to detect Cd2+ based on asymmetric PCR. In the presence of Cd2+, it can bind with Cd2+-aptamer C1 which caused the complementary strand C2 to be released from double-stranded DNA C1-C2. Because the single-stranded C1 cannot be hydrolyzed by Exo III, it can be used as a template to take part in asymmetric PCR reaction. In the absence of Cd2+, the C1-C2 was digested by Exo III and no PCR template was left. During the experiment, an interesting phenomenon was found that the asymmetric PCR can obtain higher level of fluorescent signal than that of symmetric PCR. To the best of our knowledge, this is the first report of using asymmetric PCR to detect Cd2+. Through the asymmetric PCR amplification strategy, this biosensor had a low detection limit (19.93 nM) and a wide linear range (0-500 nM). Meanwhile, this biosensor showed a satisfactory selectivity and recovery rate.
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Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Cádmio
/
Técnicas Biossensoriais
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Revista:
Spectrochim Acta A Mol Biomol Spectrosc
Assunto da revista:
BIOLOGIA MOLECULAR
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China