Cryopreservation of undifferentiated and differentiated human neuronal cells.

Yamatoya, Kenji; Nagai, Yuya; Teramoto, Naozumi; Kang, Woojin; Miyado, Kenji; Nakata, Kazuya; Yagi, Tohru; Miyamoto, Yoshitaka

Yamatoya, Kenji; Nagai, Yuya; Teramoto, Naozumi; Kang, Woojin; Miyado, Kenji; Nakata, Kazuya; Yagi, Tohru; Miyamoto, Yoshitaka.

Afiliação

Yamatoya K; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Nagai Y; Institute for Environmental and Gender-Specific Medicine, Juntendo University Graduate School of Medicine, 2-1-1 Tomioka, Urayasu, Chiba, 279-0021, Japan.
Teramoto N; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Kang W; Department of Applied Chemistry, Faculty of Engineering, Chiba Institute of Technology, 2-17-1 Tsudanuma, Narashino, Chiba, 275-0016, Japan.
Miyado K; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.
Nakata K; Department of Reproductive Biology, National Research Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo, 157-8535, Japan.
Yagi T; Department of Applied Biological Science, Faculty of Science and Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan.
Miyamoto Y; Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Technology, 2-24-16 Nakacho, Koganei, Tokyo, 184-0012, Japan.

Regen Ther ; 19: 58-68, 2022 Mar.

Article em En | MEDLINE | ID: mdl-35059480

Palavras-chave

5-CFDA-AM, 5-carboxyfluorescein diacetate; BSA, bovine serum albumin; Cryopreservation; D-MEM, Dulbecco's Modified Eagle's Medium with high glucose, l-glutamine, phenol red, and sodium pyruvate; D-PBS-free, Dulbecco's phosphate-buffered saline without Ca2+ and Mg2+ supplementation; DAPI, 4',6-Diamidino-2-phenylindole, dihydrochloride; DMSO, dimethyl sulfoxide; Differentiation; EDTA, ethylene-diamine-tetraacetic acid; ESC, embryonic stem cells; FBS, fetal bovine serum; NeuN, a neuronal marker; Neuronal cells; Regenerative therapy; SDS, sodium dodecyl sulfate; Sericin; Serum-free; iPSC, induced pluripotent stem cells

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Regen Ther Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão

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