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The DsrD functional marker protein is an allosteric activator of the DsrAB dissimilatory sulfite reductase.
Ferreira, Delfim; Barbosa, Ana C C; Oliveira, Gonçalo P; Catarino, Teresa; Venceslau, Sofia S; Pereira, Inês A C.
Afiliação
  • Ferreira D; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras 2780-156, Portugal.
  • Barbosa ACC; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras 2780-156, Portugal.
  • Oliveira GP; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras 2780-156, Portugal.
  • Catarino T; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras 2780-156, Portugal.
  • Venceslau SS; Departamento de Química, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, Caparica 2829-516, Portugal.
  • Pereira IAC; Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras 2780-156, Portugal; ipereira@itqb.unl.pt sofiasvenceslau@gmail.com.
Proc Natl Acad Sci U S A ; 119(4)2022 01 25.
Article em En | MEDLINE | ID: mdl-35064091
ABSTRACT
Dissimilatory sulfur metabolism was recently shown to be much more widespread among bacteria and archaea than previously believed. One of the key pathways involved is the dsr pathway that is responsible for sulfite reduction in sulfate-, sulfur-, thiosulfate-, and sulfite-reducing organisms, sulfur disproportionators and organosulfonate degraders, or for the production of sulfite in many photo- and chemotrophic sulfur-oxidizing prokaryotes. The key enzyme is DsrAB, the dissimilatory sulfite reductase, but a range of other Dsr proteins is involved, with different gene sets being present in organisms with a reductive or oxidative metabolism. The dsrD gene codes for a small protein of unknown function and has been widely used as a functional marker for reductive or disproportionating sulfur metabolism, although in some cases this has been disputed. Here, we present in vivo and in vitro studies showing that DsrD is a physiological partner of DsrAB and acts as an activator of its sulfite reduction activity. DsrD is expressed in respiratory but not in fermentative conditions and a ΔdsrD deletion strain could be obtained, indicating that its function is not essential. This strain grew less efficiently during sulfate and sulfite reduction. Organisms with the earliest forms of dsrAB lack the dsrD gene, revealing that its activating role arose later in evolution relative to dsrAB.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enxofre / Sulfito de Hidrogênio Redutase Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Portugal

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Enxofre / Sulfito de Hidrogênio Redutase Idioma: En Revista: Proc Natl Acad Sci U S A Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Portugal