Helitron and CACTA DNA transposons actively reshape the common wheat - AK58 genome.

ABSTRACT

Transposable elements (TEs) play a pivotal role in reshaping the plant genome. Helitrons represent a new class of transposable elements recently discovered in animals and plants. Helitrons, DNA transposons that replicate via a rolling-circle replication mechanism, are a major driving force behind genome evolution. Since the recent divergence of the modern cultivars (e.g., AK58) and landraces (e.g., Chinese Spring), Helitrons appear to have contributed greatly to genome variability. We first identified 214,665 Helitrons in AK58 by HelitronScanner software and further detected 18,668 tandem duplicated Helitron regions (TDHRs) from all the Helitrons identified. There are 39% of TDHRs (7289) translocated since the divergence of the AK58 and Chinese Spring genomes. What interested us even more are the 462 TDHRs exclusive to the AK58 genome. We also found 235 TDHRs in the 21 centromeric regions and these TDHRs contributed to centromere plasticity. Another very interesting DNA transposon, CACTA, accounting for 15% of AK58 genome, was also the focus of this study because they often inserted into gene rich regions. We found that CACTAs have inserted into many agronomically important genes, such as seed dormancy gene TaMFT and vernalization gene TaVrn1, indicating the important role of CACTAs in modern wheat adaptation.