Your browser doesn't support javascript.
loading
Exploration of Bacterial Re-Growth as In Vitro Phenomenon Affecting Methods for Analysis of the Antimicrobial Activity of Chimeric Bacteriophage Endolysins.
Kaspar, Ursula; Schleimer, Nina; Idelevich, Evgeny A; Molinaro, Sonja; Becker, Karsten.
Afiliação
  • Kaspar U; Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany.
  • Schleimer N; Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany.
  • Idelevich EA; Institute of Medical Microbiology, University Hospital Münster, 48149 Münster, Germany.
  • Molinaro S; Friedrich Loeffler-Institute of Medical Microbiology, University Medicine Greifswald, 17475 Greifswald, Germany.
  • Becker K; Microcoat Biotechnologie GmbH, 82347 Bernried, Germany.
Microorganisms ; 10(2)2022 Feb 15.
Article em En | MEDLINE | ID: mdl-35208898
ABSTRACT
Drug alternatives to combat methicillin-resistant Staphylococcus aureus (MRSA) in human and animal healthcare are urgently needed. Recently, the recombinant bacteriophage endolysins, PRF-119 and its successor substance HY-133, have proven to be highly active against various S. aureus clonal lineages and to exhibit a very rapid bactericidal effect when standard methods for susceptibility testing are applied. Along with subsequent growth curve experiments, a re-growth phenomenon was observed in vitro necessitating its clarification for the assessment of the agent's stability and activity as well as for methodological aspects of endolysin testing in general. Distinct in vitro parameters were comparatively examined applying also scanning electron microscopy, fluorescence assays and SDS-PAGE analysis. The shape and material of the culture vessels as well as the shaking conditions were identified as factors influencing the in vitro stability and activity of HY-133. The highest function maintenance was observed in plain centrifuge tubes. Based on this, the conditions and parameters of assays for testing the antimicrobial activities of phage endolysins were determined and adjusted. In particular, shear forces should be kept to a minimum. Our results form the basis for both future test standardization and re-growth-independent experiments as prerequisites for exact determination of the antimicrobial activities of engineered endolysins.
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Microorganisms Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Alemanha