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Endocrine, immune and renal toxicity in male largemouth bass after chronic exposure to glyphosate and Rodeo®.
De Maria, Maite; Kroll, Kevin J; Yu, Fahong; Nouri, Mohammad-Zaman; Silva-Sanchez, Cecilia; Perez, Juan Guillermo; Moraga Amador, David A; Zhang, Yanping; Walsh, Mike T; Denslow, Nancy D.
Afiliação
  • De Maria M; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States. Electronic address: maitedmm@gmail.com.
  • Kroll KJ; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States.
  • Yu F; Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, Gainesville, FL 32611, United States.
  • Nouri MZ; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States.
  • Silva-Sanchez C; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States.
  • Perez JG; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States.
  • Moraga Amador DA; Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, Gainesville, FL 32611, United States.
  • Zhang Y; Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida, Gainesville, FL 32611, United States.
  • Walsh MT; Aquatic Animal Health Program, College of Veterinary Medicine, University of Florida, PO Box 100136, Gainesville, FL 32610, United States.
  • Denslow ND; Department of Physiological Sciences and Center for Environmental and Human Toxicology, University of Florida, Gainesville, FL 32611, United States; Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, United States. Electronic address: ndenslow@ufl.edu.
Aquat Toxicol ; 246: 106142, 2022 May.
Article em En | MEDLINE | ID: mdl-35306440
ABSTRACT
Glyphosate is the most used herbicide worldwide, with no historical comparison. It is used for genetically modified crops, and particularly in Florida, it is used as a sugar cane ripener. An aquatic formulation (Rodeo®) is used to treat aquatic weeds in waterbodies and drainage canals. Because of its extended use, glyphosate can run off or be sprayed directly into waterbodies, and chronically expose aquatic wildlife. Exposure in animal models has been associated with kidney and liver damage and glyphosate has been suggested as an endocrine disruptor. We exposed adult male largemouth bass for 21 days to two doses of glyphosate and Rodeo® (chemically equivalent concentration of glyphosate) at 0.5 mg L-1 and 10 mg L-1 and to a clean water control (n=4 fish/tank in quadruplicate). Concentrations during the experiment were corroborated with UHPLC-MS/MS. Total RNA was isolated from the trunk kidney and head kidney. RNA-seq was performed for the high doses compared to controls. Transcripts were analyzed with fish and mammalian pathway analyses software. Transcripts mapped to Zebrafish metabolic pathways using PaintOmics showed steroid hormone biosynthesis in the trunk kidney as the most significantly enriched pathway. Steroid hormones were measured in plasma by UHPLC-MS/MS. Total androgens were significantly reduced at 0.5 mg L-1 of glyphosate and at equivalent concentrations in Rodeo® compared to controls. 11-ketotestosterone and estrone concentrations were significantly reduced in all doses. A gene involved in the conversion of testosterone to 11-ketotestosterone was down-regulated by glyphosate. Using the mammalian pathway analysis algorithm, cellular processes associated with T-cell activation/development and intracellular pH were significantly enriched in the trunk kidney by glyphosate and Rodeo® exposure. Endocrine disruption was corroborated at the hormone and gene expression levels. Rodeo® and glyphosate share gene expression pathways, however, Rodeo® had more pronounced effects in largemouth bass.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Bass / Herbicidas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Aquat Toxicol Assunto da revista: BIOLOGIA / TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Bass / Herbicidas Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Aquat Toxicol Assunto da revista: BIOLOGIA / TOXICOLOGIA Ano de publicação: 2022 Tipo de documento: Article