Streptomyces gobiensis sp. nov., an antimicrobial producing actinobacterium isolated from soil under black Gobi rocks.
Int J Syst Evol Microbiol
; 72(3)2022 Mar.
Article
em En
| MEDLINE
| ID: mdl-35320067
ABSTRACT
A novel actinomycete, strain 1_25T, was isolated from soil under a black Gobi rock sample from Shuangta, PR China, and characterized using a polyphasic taxonomic approach. The results of comparative analysis of the 16S rRNA gene sequences indicated the 1_25T represented a member of the genus Streptomyces. Chemotaxonomic data revealed that 1_25T possessed MK-9(H8) as the major menaquinone. The cell wall contained ll-diaminopimelic acid (ll-DAP) and the whole-cell sugar pattern consisted of ribose, glucose and galactose. Major fatty acid methyl esters were observed to be iso-C16ââ0 (23.6â%), and anteiso-C15ââ0 (10.4â%). The genomic DNA G+C content of 1_25T was 69 molâ%. The results of phylogenetic analysis based on 16S rRNA gene sequence indicated that 1_25T had high sequence similarity with Streptomyces qinglanensis 172205T (98.1â%), Streptomyces lycii TRM 66187T (98â%), and Streptomyces griseocarneus JCM4580T (98â%). In addition to the differences in phenotypic characters, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between 1_25T and closely related species were below the recommended threshold values for assigning strains to the same species. The fermentation product of 1_25T in ISP2 had an inhibitory effect on Staphylococcus aureus. On the basis of these genotypic and phenotypic characteristics, strain 1_25T (=JCM 34936T=GDMCC 4.216T) represents a novel species of the genus Streptomyces, for which the name Streptomyces gobiensis sp. nov. is proposed.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Streptomyces
/
Actinobacteria
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Anti-Infecciosos
Idioma:
En
Revista:
Int J Syst Evol Microbiol
Assunto da revista:
MICROBIOLOGIA
Ano de publicação:
2022
Tipo de documento:
Article