Optimized APEX2 peroxidase-mediated proximity labeling in fast- and slow-growing mycobacteria.

Ahamed, Mukshud; Jaisinghani, Neetika; Li, Michael; Winkeler, Ian; Silva, Shalika; Previti, Mary L; Seeliger, Jessica C

Ahamed, Mukshud; Jaisinghani, Neetika; Li, Michael; Winkeler, Ian; Silva, Shalika; Previti, Mary L; Seeliger, Jessica C.

Afiliação

Ahamed M; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Jaisinghani N; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Li M; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Winkeler I; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Silva S; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Previti ML; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States.
Seeliger JC; Department of Pharmacological Sciences, Stony Brook University, Stony Brook, NY, United States. Electronic address: jessica.seeliger@stonybrook.edu.

Methods Enzymol ; 664: 267-289, 2022.

Article em En | MEDLINE | ID: mdl-35331378

Assuntos

Mycobacterium; Peroxidase; Corantes; Citoplasma/metabolismo; DNA Liase (Sítios Apurínicos ou Apirimidínicos)/genética; DNA Liase (Sítios Apurínicos ou Apirimidínicos)/metabolismo; Endonucleases/metabolismo; Enzimas Multifuncionais/metabolismo; Mycobacterium/genética; Mycobacterium/metabolismo; Proteoma/metabolismo

Palavras-chave

APEX2; Mycobacteria; Periplasm; Peroxidase; Protein labeling; Proximity labeling; Tuberculosis

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Peroxidase / Mycobacterium Idioma: En Revista: Methods Enzymol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos

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