Human neutrophils are resistant to Clostridioides difficile toxin B.
Anaerobe
; 74: 102553, 2022 Apr.
Article
em En
| MEDLINE
| ID: mdl-35346824
ABSTRACT
OBJECTIVE:
The main objective of this study was to evaluate the glucosyltransferase activity of C. difficile TcdB on the activity of human PMNs.METHODS:
To better understand the interaction between PMNs and TcdB, PMNs were treated with sub-lethal concentrations of TcdB. We evaluated (i) the glucosylation of GTPases, (ii) the phagocytic and bactericidal activity, and (iii) PMNs activation (through quantification of TNF-α, IL-8, and expression of CD11b cell surface activation marker).RESULTS:
We found that TcdB did not glucosylate RhoA and Rac1 GTPases and did not affect the phagocytic or bactericidal capacity of PMNs. Moreover, TcdB did not increase the production of TNF-α, IL-8, or the expression of activation marker CD11b. The only significant effect of TcdB on PMNs was the partial inhibition of TNF-α and IL-8 production and the diminished expression of CD11b induced by E. coli-LPS.CONCLUSION:
Our results show that human PMNs are resistant to TcdB GTPase glucosyltransferase activity against RhoA and Rac1.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Clostridioides difficile
Limite:
Humans
Idioma:
En
Revista:
Anaerobe
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Costa Rica