Live-Cell Fluorescence Lifetime Multiplexing Using Synthetic Fluorescent Probes.
ACS Chem Biol
; 17(6): 1321-1327, 2022 06 17.
Article
em En
| MEDLINE
| ID: mdl-35584304
ABSTRACT
Fluorescence lifetime multiplexing requires fluorescent probes with distinct fluorescence lifetimes but similar spectral properties. Even though synthetic probes for many cellular targets are available for multicolor live-cell fluorescence microscopy, few of them have been characterized for their use in fluorescence lifetime multiplexing. Here, we demonstrate that, from a panel of 18 synthetic probes, eight pairwise combinations are suitable for fluorescence lifetime multiplexing in living mammalian cell lines. Moreover, combining multiple pairs in different spectral channels enables us to image four and with the help of self-labeling protein tags up to eight different biological targets, effectively doubling the number of observable targets. The combination of synthetic probes with fluorescence lifetime multiplexing is thus a powerful approach for live-cell imaging.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Corantes Fluorescentes
/
Mamíferos
Limite:
Animals
Idioma:
En
Revista:
ACS Chem Biol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Alemanha