The UPRmt preserves mitochondrial import to extend lifespan.
J Cell Biol
; 221(7)2022 07 04.
Article
em En
| MEDLINE
| ID: mdl-35608535
ABSTRACT
The mitochondrial unfolded protein response (UPRmt) is dedicated to promoting mitochondrial proteostasis and is linked to extreme longevity. The key regulator of this process is the transcription factor ATFS-1, which, upon UPRmt activation, is excluded from the mitochondria and enters the nucleus to regulate UPRmt genes. However, the repair proteins synthesized as a direct result of UPRmt activation must be transported into damaged mitochondria that had previously excluded ATFS-1 owing to reduced import efficiency. To address this conundrum, we analyzed the role of the import machinery when the UPRmt was induced. Using in vitro and in vivo analysis of mitochondrial proteins, we surprisingly find that mitochondrial import increases when the UPRmt is activated in an ATFS-1-dependent manner, despite reduced mitochondrial membrane potential. The import machinery is upregulated, and an intact import machinery is essential for UPRmt-mediated lifespan extension. ATFS-1 has a weak mitochondrial targeting sequence (MTS), allowing for dynamic subcellular localization during the initial stages of UPRmt activation.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Fatores de Transcrição
/
Proteínas de Caenorhabditis elegans
/
Resposta a Proteínas não Dobradas
/
Longevidade
/
Mitocôndrias
Limite:
Animals
Idioma:
En
Revista:
J Cell Biol
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Canadá