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Sabatier Principle for Rationalizing Enzymatic Hydrolysis of a Synthetic Polyester.
Arnling Bååth, Jenny; Jensen, Kenneth; Borch, Kim; Westh, Peter; Kari, Jeppe.
Afiliação
  • Arnling Bååth J; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Kgs. Lyngby DK-2800, Denmark.
  • Jensen K; Novozymes A/S, Biologiens Vej 2, Kgs. Lyngby DK-2800, Denmark.
  • Borch K; Novozymes A/S, Biologiens Vej 2, Kgs. Lyngby DK-2800, Denmark.
  • Westh P; Department of Biotechnology and Biomedicine, Technical University of Denmark, Søltofts Plads, Kgs. Lyngby DK-2800, Denmark.
  • Kari J; Department of Science and Environment, Roskilde University, Universitetsvej 1, Roskilde DK-4000, Denmark.
JACS Au ; 2(5): 1223-1231, 2022 May 23.
Article em En | MEDLINE | ID: mdl-35647598
Interfacial enzyme reactions are common in Nature and in industrial settings, including the enzymatic deconstruction of poly(ethylene terephthalate) (PET) waste. Kinetic descriptions of PET hydrolases are necessary for both comparative analyses, discussions of structure-function relations and rational optimization of technical processes. We investigated whether the Sabatier principle could be used for this purpose. Specifically, we compared the kinetics of two well-known PET hydrolases, leaf-branch compost cutinase (LCC) and a cutinase from the bacterium Thermobifida fusca (TfC), when adding different concentrations of the surfactant cetyltrimethylammonium bromide (CTAB). We found that CTAB consistently lowered the strength of enzyme-PET interactions, while its effect on enzymatic turnover was strongly biphasic. Thus, at gradually increasing CTAB concentrations, turnover was initially promoted and subsequently suppressed. This correlation with maximal turnover at an intermediate binding strength was in accordance with the Sabatier principle. One consequence of these results was that both enzymes had too strong intrinsic interaction with PET for optimal turnover, especially TfC, which showed a 20-fold improvement of k cat at the maximum. LCC on the other hand had an intrinsic substrate affinity closer to the Sabatier optimum, and the turnover rate was 5-fold improved at weakened substrate binding. Our results showed that the Sabatier principle may indeed rationalize enzymatic PET degradation and support process optimization. Finally, we suggest that future discovery efforts should consider enzymes with weakened substrate binding because strong adsorption seems to limit their catalytic performance.

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: JACS Au Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Dinamarca

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: JACS Au Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Dinamarca