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A reference tissue atlas for the human kidney.
Hansen, Jens; Sealfon, Rachel; Menon, Rajasree; Eadon, Michael T; Lake, Blue B; Steck, Becky; Anjani, Kavya; Parikh, Samir; Sigdel, Tara K; Zhang, Guanshi; Velickovic, Dusan; Barwinska, Daria; Alexandrov, Theodore; Dobi, Dejan; Rashmi, Priyanka; Otto, Edgar A; Rivera, Miguel; Rose, Michael P; Anderton, Christopher R; Shapiro, John P; Pamreddy, Annapurna; Winfree, Seth; Xiong, Yuguang; He, Yongqun; de Boer, Ian H; Hodgin, Jeffrey B; Barisoni, Laura; Naik, Abhijit S; Sharma, Kumar; Sarwal, Minnie M; Zhang, Kun; Himmelfarb, Jonathan; Rovin, Brad; El-Achkar, Tarek M; Laszik, Zoltan; He, John Cijiang; Dagher, Pierre C; Valerius, M Todd; Jain, Sanjay; Satlin, Lisa M; Troyanskaya, Olga G; Kretzler, Matthias; Iyengar, Ravi; Azeloglu, Evren U.
Afiliação
  • Hansen J; Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Sealfon R; Princeton University, Princeton, NJ, USA.
  • Menon R; Flatiron Institute, New York, NY, USA.
  • Eadon MT; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Lake BB; Indiana University School of Medicine, Indianapolis, IN, USA.
  • Steck B; Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.
  • Anjani K; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Parikh S; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Sigdel TK; Ohio State University College of Medicine, Columbus, OH, USA.
  • Zhang G; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Velickovic D; University of Texas-Health San Antonio School of Medicine, San Antonio, TX, USA.
  • Barwinska D; Pacific Northwest National Laboratory, Richland, WA, USA.
  • Alexandrov T; Indiana University School of Medicine, Indianapolis, IN, USA.
  • Dobi D; European Molecular Biology Laboratory, Heidelberg, Germany.
  • Rashmi P; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Otto EA; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Rivera M; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Rose MP; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Anderton CR; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Shapiro JP; University of Texas-Health San Antonio School of Medicine, San Antonio, TX, USA.
  • Pamreddy A; Pacific Northwest National Laboratory, Richland, WA, USA.
  • Winfree S; Ohio State University College of Medicine, Columbus, OH, USA.
  • Xiong Y; University of Texas-Health San Antonio School of Medicine, San Antonio, TX, USA.
  • He Y; Indiana University School of Medicine, Indianapolis, IN, USA.
  • de Boer IH; Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Hodgin JB; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Barisoni L; Schools of Medicine and Public Health, University of Washington, Seattle, WA, USA.
  • Naik AS; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Sharma K; Duke University School of Medicine, Durham, NC, USA.
  • Sarwal MM; University of Michigan School of Medicine, Ann Arbor, MI, USA .
  • Zhang K; University of Texas-Health San Antonio School of Medicine, San Antonio, TX, USA.
  • Himmelfarb J; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Rovin B; Department of Bioengineering, University of California San Diego, La Jolla, CA, USA.
  • El-Achkar TM; Schools of Medicine and Public Health, University of Washington, Seattle, WA, USA.
  • Laszik Z; Ohio State University College of Medicine, Columbus, OH, USA.
  • He JC; Indiana University School of Medicine, Indianapolis, IN, USA.
  • Dagher PC; University of California San Francisco School of Medicine, San Francisco, CA, USA.
  • Valerius MT; Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Jain S; Indiana University School of Medicine, Indianapolis, IN, USA.
  • Satlin LM; Brigham and Women's Hospital, Harvard Medical School, Cambridge, MA, USA.
  • Troyanskaya OG; Washington University in Saint Louis School of Medicine, St. Louis, MS, USA.
  • Kretzler M; Icahn School of Medicine at Mount Sinai, New York, NY, USA.
  • Iyengar R; Princeton University, Princeton, NJ, USA.
  • Azeloglu EU; Flatiron Institute, New York, NY, USA.
Sci Adv ; 8(23): eabn4965, 2022 06 10.
Article em En | MEDLINE | ID: mdl-35675394
ABSTRACT
Kidney Precision Medicine Project (KPMP) is building a spatially specified human kidney tissue atlas in health and disease with single-cell resolution. Here, we describe the construction of an integrated reference map of cells, pathways, and genes using unaffected regions of nephrectomy tissues and undiseased human biopsies from 56 adult subjects. We use single-cell/nucleus transcriptomics, subsegmental laser microdissection transcriptomics and proteomics, near-single-cell proteomics, 3D and CODEX imaging, and spatial metabolomics to hierarchically identify genes, pathways, and cells. Integrated data from these different technologies coherently identify cell types/subtypes within different nephron segments and the interstitium. These profiles describe cell-level functional organization of the kidney following its physiological functions and link cell subtypes to genes, proteins, metabolites, and pathways. They further show that messenger RNA levels along the nephron are congruent with the subsegmental physiological activity. This reference atlas provides a framework for the classification of kidney disease when multiple molecular mechanisms underlie convergent clinical phenotypes.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rim / Nefropatias Limite: Humans Idioma: En Revista: Sci Adv Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Rim / Nefropatias Limite: Humans Idioma: En Revista: Sci Adv Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Estados Unidos