Your browser doesn't support javascript.
loading
Tagging and Capture of Prenylated CaaX-Proteins from Plant Cell Cultures.
Ribeiro, Iliana; Ducos, Eric; Giglioli-Guivarc'h, Nathalie; Dutilleul, Christelle.
Afiliação
  • Ribeiro I; EA2106 "Biomolécules et Biotechnologies Végétales", Faculté des Sciences Pharmaceutiques, Université de Tours, Parc de Grandmont, Tours, France.
  • Ducos E; EA2106 "Biomolécules et Biotechnologies Végétales", Faculté des Sciences Pharmaceutiques, Université de Tours, Parc de Grandmont, Tours, France.
  • Giglioli-Guivarc'h N; EA2106 "Biomolécules et Biotechnologies Végétales", Faculté des Sciences Pharmaceutiques, Université de Tours, Parc de Grandmont, Tours, France.
  • Dutilleul C; EA2106 "Biomolécules et Biotechnologies Végétales", Faculté des Sciences Pharmaceutiques, Université de Tours, Parc de Grandmont, Tours, France. christelle.dutilleul@univ-tours.fr.
Methods Mol Biol ; 2505: 241-248, 2022.
Article em En | MEDLINE | ID: mdl-35732949
The tagging-via-substrate strategy allows the probing of in vivo post-translationally modified proteins thanks to a labeled substrate. This method has been used for the detection and proteomic analysis of prenylated proteins in mammals and more recently in plants. It consists of the labeling of prenylated proteins by supplying azido-prenyl to cells. The azido-prenylated proteins are then selectively linked to biotin alkyne, which allows their capture using streptavidin beads, and their subsequent identification by mass spectrometry. In this chapter, we describe this procedure on Arabidopsis cell suspension and how it can be applied for Catharanthus roseus cells.
Assuntos
Palavras-chave

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteômica Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Arabidopsis / Proteômica Limite: Animals Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2022 Tipo de documento: Article País de afiliação: França