Accurate Ensemble Prediction of Somatic Mutations with SMuRF2.

ABSTRACT

Accurate identification of somatic mutations is crucial for discovery and identification of driver mutations in cancer tumors. Here, we describe the updated Somatic Mutation calling method using a Random Forest (SMuRF2), an ensemble method that combines the predictions and auxiliary features from individual mutation callers using supervised machine learning. SMuRF2 provides an efficient workflow to predict both somatic point mutations (SNVs) and small insertions/deletions (indels) in cancer genomes and exomes. We describe the latest method and provide a detailed tutorial for running SMuRF2.