A root phloem pole cell atlas reveals common transcriptional states in protophloem-adjacent cells.
Nat Plants
; 8(8): 954-970, 2022 08.
Article
em En
| MEDLINE
| ID: mdl-35927456
ABSTRACT
Single-cell sequencing has recently allowed the generation of exhaustive root cell atlases. However, some cell types are elusive and remain underrepresented. Here we use a second-generation single-cell approach, where we zoom in on the root transcriptome sorting with specific markers to profile the phloem poles at an unprecedented resolution. Our data highlight the similarities among the developmental trajectories and gene regulatory networks common to protophloem sieve element (PSE)-adjacent lineages in relation to PSE enucleation, a key event in phloem biology. As a signature for early PSE-adjacent lineages, we have identified a set of DNA-binding with one finger (DOF) transcription factors, the PINEAPPLEs (PAPL), that act downstream of PHLOEM EARLY DOF (PEAR) genes and are important to guarantee a proper root nutrition in the transition to autotrophy. Our data provide a holistic view of the phloem poles that act as a functional unit in root development.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Arabidopsis
/
Floema
Tipo de estudo:
Prognostic_studies
Idioma:
En
Revista:
Nat Plants
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
Reino Unido