Complete genomic sequence analysis and intestinal tissue localization of a porcine Kobuvirus variant in China.
Infect Genet Evol
; 104: 105362, 2022 10.
Article
em En
| MEDLINE
| ID: mdl-36084837
ABSTRACT
Porcine kobuvirus (PKV) infection is very common in both healthy pigs and diarrhea pigs throughout the world. However, there is no proof that it causes diarrhea, and little is known about its role in diarrhea. There are only a few reports concerning porcine kobuvirus separation at present, which makes investigating its invasion and pathogenesis mechanisms difficult. This study sequenced the entire genome of a porcine kobuvirus strain termed "Wuhan2020" after it was isolated from intestinal tissue samples of healthy piglets. The analysis results revealed that it shared the most resemblance with the WUH1 strain (89.5%) and belonged to the same evolutionary branch as the Hungarian strain S-1-SUN. The PKV was located using the in situ hybridization (ISH) approach, which revealed that it was colonized in intestinal villus epithelial cells and lymphocytes in the Peyer's patch. In general, we analyzed the genetic evolution of PKV, discovered PKV susceptible cells and determined PKV localization in the intestine of infected pigs, providing a reference for future research.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Doenças dos Suínos
/
Infecções por Picornaviridae
/
Kobuvirus
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
Infect Genet Evol
Assunto da revista:
BIOLOGIA
/
DOENCAS TRANSMISSIVEIS
/
GENETICA
Ano de publicação:
2022
Tipo de documento:
Article