Multifunctional-high resolution imaging plate based on hydrophilic graphene for digital pathology.
Nanotechnology
; 33(50)2022 Oct 03.
Article
em En
| MEDLINE
| ID: mdl-36095982
ABSTRACT
In the present study, we showed that hydrophilic graphene can serve as an ideal imaging plate for biological specimens. Graphene being a single-atom-thick semi-metal with low secondary electron emission, array tomography analysis of serial sections of biological specimens on a graphene substrate showed excellent image quality with improvedz-axis resolution, without including any conductive surface coatings. However, the hydrophobic nature of graphene makes the placement of biological specimens difficult; graphene functionalized with polydimethylsiloxane oligomer was fabricated using a simple soft lithography technique and then processed with oxygen plasma to provide hydrophilic graphene with minimal damage to graphene. High-quality scanning electron microscopy images of biological specimens free from charging effects or distortion were obtained, and the optical transparency of graphene enabled fluorescence imaging of the specimen; high-resolution correlated electron and light microscopy analysis of the specimen became possible with the hydrophilic graphene plate.
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01-internacional
Base de dados:
MEDLINE
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Grafite
Idioma:
En
Revista:
Nanotechnology
Ano de publicação:
2022
Tipo de documento:
Article