Metabolic Engineering for the Comprehensive Utilization of N,N-Dimethylformamide-Containing Wastewater.
J Agric Food Chem
; 70(42): 13574-13582, 2022 Oct 26.
Article
em En
| MEDLINE
| ID: mdl-36223298
ABSTRACT
N, N-dimethylformamide is frequently present in industrial wastewater and is environmentally detrimental. The current study aims to assess the utilization and biodegradation of N, N-dimethylformamide-containing wastewater to lessen the associated environmental load. Results show that addition of wastewater containing N, N-dimethylformamide to Trichoderma reesei fermentation media enhances cellulase production and facilitates cellulose hydrolysis. However, N, N-dimethylformamide is a cellulase enhancer that is not degraded during cellulase production in T. reesei fermentation and is retained in the N, N-dimethylformamide-enhanced cellulase solution. Indeed, the cellulosic sugar solution generated via lignocellulose hydrolysis with N, N-dimethylformamide-enhanced cellulase retains N, N-dimethylformamide. We further identified three core enzyme modulesâN, N-dimethylformamidase, dimethylamine dehydrogenase, and methylamine dehydrogenase enzymeâwhich were inserted into Escherichia coli to develop metabolically engineered strains. These strains degraded N, N-dimethylformamide and produced succinate using N, N-dimethylformamide-enhanced cellulosic sugar as the substrate. The platform described here can be applied to effectively convert waste into valuable bioproducts.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Trichoderma
/
Celulase
Idioma:
En
Revista:
J Agric Food Chem
Ano de publicação:
2022
Tipo de documento:
Article
País de afiliação:
China