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Bacterial Diversity Associated with Anastrepha obliqua Males Change Under Mass-Rearing Conditions and with Irradiation.
Roque-Romero, Linnet; Guillén-Navarro, Karina; Zarza, Eugenia; Montoya, Pablo; Liedo, Pablo.
Afiliação
  • Roque-Romero L; El Colegio de La Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico. linnet.roque@estudianteposgrado.ecosur.mx.
  • Guillén-Navarro K; El Colegio de La Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Zarza E; El Colegio de La Frontera Sur (ECOSUR), Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Montoya P; Consejo Nacional de Ciencia Y Tecnología (Investigadora Por Mexico), commissioned to El Colegio de La Frontera Sur, Carretera Antiguo Aeropuerto Km 2.5, 30700, Tapachula, Chiapas, Mexico.
  • Liedo P; Programa Moscas de La Fruta SENASICA-SADER, Camino a los Cacaotales S/N, C.P. 30860, Metapa de Domínguez, Chiapas, Mexico.
Curr Microbiol ; 80(1): 26, 2022 Dec 06.
Article em En | MEDLINE | ID: mdl-36474118
ABSTRACT
Recent advances in understanding the symbiotic interactions between bacteria and fruit flies have shown that they are relevant for mass rearing and the sterile insect technique (SIT). SIT involves mass production and release of sterile insects that would copulate with their wild conspecifics and thus decrease the population growth rate. The irradiation process used to sterilize mass-reared flies can modify the diversity and structure of the midgut bacterial communities, which could affect sterile male survival, flight capacity, and sexual competitiveness. Our aim was to compare bacterial communities in the midgut of wild and mass-reared Anastrepha obliqua (Macquart) males irradiated at 0, 60, and 80 Gy. After adult's emergence, their midguts were dissected, DNA was extracted, and high-throughput sequencing of the V3-V4 region of the 16S rDNA gene was performed. A total of 11 phyla, 17 classes, 47 families, and 52 genera of bacteria were identified. The most representative phylum was Proteobacteria and the predominant family was Enterobacteriaceae. We found that wild males had a different intestinal bacterial community from mass-reared males. In addition, irradiation at 60 and 80 Gy caused changes in the diversity and structure of the midgut microbiota of these sterile males, suggesting that mass rearing and irradiation cause artificial selection of the bacterial communities in the gut of A. obliqua males.
Assuntos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Curr Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Curr Microbiol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México