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Ratiometric Sensing of Intracellular pH Based on Dual Emissive Carbon Dots.
Sun, Yanli; Wang, Qin; Liu, Jin; Zhao, Zuoping; Li, Lihua; Liu, Zhifeng; Lu, Jiufu; Jin, Lingxia; Zhang, Shengrui.
Afiliação
  • Sun Y; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Wang Q; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China. qincaimuxu009@sina.cn.
  • Liu J; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Zhao Z; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Li L; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Liu Z; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Lu J; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Jin L; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China.
  • Zhang S; Shaanxi Key Laboratory of Catalysis, School of Chemistry and Environment Science, Shaanxi University of Technology, 723000, Hanzhong, Shaanxi, China. zhangshr09@sina.com.
J Fluoresc ; 33(2): 653-661, 2023 Mar.
Article em En | MEDLINE | ID: mdl-36480126
ABSTRACT
Accurate monitoring of intracellular pH in living cells is critical for developing a better understanding of cellular activities. In the current study, label-free carbon dots (p-CDs), which were fabricated using a straightforward one-pot solvothermal treatment of p-phenylenediamine and urea, were employed to create a new ratiometric pH nanosensor. Under single-wavelength excitation (λex = 500 nm), the p-CDs gave dual emission bands at 525 and 623 nm. The fluorescent intensity ratio (I525/I623) was linearly related to pH over the range 4.0 to 8.8 in buffer solutions, indicating that the ratiometric fluorescence nanoprobe may be useful for pH sensing. In pH measurements, the p-CDs also demonstrated outstanding selectivity, reversibility, and photostability. Owing to the advantages outlined above, the nanoprobe was used to monitor the pH of HeLa cells effectively. The label-free CD-based ratiometric nanoprobe features comparatively easy manufacturing and longer excitation and emission wavelengths than the majority of previously reported CD-based ratiometric pH sensors, which is ultimately beneficial for applications in biological imaging.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pontos Quânticos Limite: Humans Idioma: En Revista: J Fluoresc Assunto da revista: BIOFISICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pontos Quânticos Limite: Humans Idioma: En Revista: J Fluoresc Assunto da revista: BIOFISICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China