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Optical Spectrophotometry as a Promising Method for Quantification and Stage Differentiation of Plasmodium falciparum Parasites.
Baptista, Vitória; Silva, Miguel; Ferreira, Gabriel M; Calçada, Carla; Minas, Graça; Veiga, Maria Isabel; Catarino, Susana O.
Afiliação
  • Baptista V; Microelectromechanical Systems Research Unit (CMEMS-UMinho), School of Engineering, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.
  • Silva M; LABBELS─Associate Laboratory, 4800-058 Braga/Guimarães, Portugal.
  • Ferreira GM; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
  • Calçada C; ICVS/3B's─PT Government Associate Laboratory, 4806-909 Guimarães, Braga/, Portugal.
  • Minas G; Life and Health Sciences Research Institute (ICVS), School of Medicine, University of Minho, Campus de Gualtar, 4710-057 Braga, Portugal.
  • Veiga MI; ICVS/3B's─PT Government Associate Laboratory, 4806-909 Guimarães, Braga/, Portugal.
  • Catarino SO; Microelectromechanical Systems Research Unit (CMEMS-UMinho), School of Engineering, University of Minho, Campus de Azurém, 4800-058 Guimarães, Portugal.
ACS Infect Dis ; 9(1): 140-149, 2023 01 13.
Article em En | MEDLINE | ID: mdl-36490289
ABSTRACT
Malaria is one of the most life-threatening infectious diseases worldwide, claiming half a million lives yearly. Prompt and accurate diagnosis is crucial for disease control and elimination. Currently used diagnostic methods require blood sampling and fail to detect low-level infections. At the symptomatic stage of infection, the parasites feed on red blood cells' (RBCs) hemoglobin, forming inert crystals, the hemozoin, in the process. Thus, along with parasite maturation inside the RBCs, the hemoglobin and hemozoin proportion is inversely related, and they generate specific optical spectra, according to their concentration. Herein, to address the issues of finger prick sampling and the lack of sensitivity of the parasitological test, we explored the optical features of Plasmodium falciparum-infected RBCs through absorbance and reflectance spectrophotometric characterization, aiming for their detection. This is the first work fully characterizing the spectrophotometric properties of P. falciparum-infected RBCs by using only 16 specific wavelengths within the visible optical spectra and two different post-processing algorithms. With such an innovative methodology, low-level infections can be detected and quantified, and early- and late-stage development can be clearly distinguished, not only improving the current detection limits but also proving the successful applicability of spectrophotometry for competitive and accurate malaria diagnosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parasitos / Malária Falciparum / Malária Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: ACS Infect Dis Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Portugal

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Parasitos / Malária Falciparum / Malária Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: ACS Infect Dis Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Portugal