A simplified method for detecting Gram-positive and Gram-negative bacteria in dental histological samples: A preliminary and comparative study.
Acta Histochem
; 125(1): 151992, 2023 Jan.
Article
em En
| MEDLINE
| ID: mdl-36566583
ABSTRACT
OBJECTIVE:
Bacteria identification inside the dental tissue is a complex procedure requiring specific protocols. This study aimed to compare two classical Gram staining methods with a new staining method proposed by the authors to detect Gram-positive and Gram-negative bacteria in dental histological samples of human dentin.METHODS:
Ten human teeth, extracted because of various pathologies, were decalcified, dehydrated, and paraffin-embedded. Then, approximately 100 serial sections of 4 µm thickness were made per sample. The serial sections were placed on glass slides and were stained according to Brown-Brenn, Brown-Hopps, and a proposed modification of Brown-Brenn staining. Both ATCC strains, smeared on glass slides, were stained following each method's instructions used in histological samples.RESULTS:
From a qualitative evaluation, the Brown-Brenn method resulted in better staining of Gram-positive bacteria, while the authors' proposed staining technique was more oriented towards Gram-negative bacteria. On the other hand, the Brown-Hopps showed quite a balance in detecting Gram-positive and Gram-negative bacteria. Unlike the Brown-Brenn stain, the other two protocols showed better stainability of Gram-negative microorganisms in bacterial-smeared samples.CONCLUSION:
All staining techniques evaluated in this article can identify bacteria, but the outcome can change according to the staining procedure used.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Bactérias Gram-Negativas
/
Bactérias Gram-Positivas
Tipo de estudo:
Qualitative_research
Limite:
Humans
Idioma:
En
Revista:
Acta Histochem
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Itália