Testing the susceptibility of larval stages of Simulium to temephos and Bacillus thuringiensis var israelensis in Germany and Northern Cameroon.
Med Vet Entomol
; 37(2): 286-299, 2023 06.
Article
em En
| MEDLINE
| ID: mdl-36571295
ABSTRACT
Assays to evaluate the susceptibility of Simulium larvae to temephos and Bacillus thuringiensis var israelensis (Bti) were carried out by setting-up an in vitro laboratory test ('bio assay') and a semi-natural test ('système de goutières') to assess the LC50/LC90 values. Larvae of Simulium species in Cameroon (S. damnosum s.l., S. hargreavesi, S. vorax and S. cervicornutum) and (S. (Odagmia) ornatum and S. latipes) in Germany were identified and tested. In the bio-assay, 50 larvae were exposed for 10 min to concentrations from 0.01 to 10 ppm. For the Simulium from Germany, the LC50 (LC90) values after 3 and 6 h were 3.1 (27.9) and 0.14 (1.26) ppm for temephos and for Bti 7.8 (70.2) and 1.7 (15.3) ppm, respectively. For Cameroonian species, the values of LC50 (LC90) were lower, that is, 0.42 (8.04), 0.14 (2.70) and 0.073 (1.38) ppm, respectively, after 3, 6 and 12 h for temephos. In a semi natural condition, the LC50 of 10 min of application of temephos was 0.84 ppm after 3 h and a working solution (2.6 L) of Bti killed 50% after 6 h. To detect an upcoming of any resistance as it happened in Ivory Coast, a study of the occurrence resistance genes should be implemented.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Simuliidae
/
Bacillus thuringiensis
/
Inseticidas
Limite:
Animals
País/Região como assunto:
Africa
/
Europa
Idioma:
En
Revista:
Med Vet Entomol
Assunto da revista:
BIOLOGIA
/
MEDICINA VETERINARIA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Alemanha