Mass Spectrometry of Neutral Glycosphingolipids.

Suzuki, Akemi; Silsirivanit, Atit; Watanabe, Takashi; Matsuda, Junko; Inamori, Kei-Ichiro; Inokuchi, Jin-Ichi

Suzuki, Akemi; Silsirivanit, Atit; Watanabe, Takashi; Matsuda, Junko; Inamori, Kei-Ichiro; Inokuchi, Jin-Ichi.

Afiliação

Suzuki A; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan. akmszk@gaea.ocn.ne.jp.
Silsirivanit A; Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
Watanabe T; Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
Matsuda J; Department of Pathophysiology and Metabolism, Kawasaki Medical School, Okayama, Japan.
Inamori KI; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.
Inokuchi JI; Institute of Molecular Biomembrane and Glycobiology, Tohoku Medical and Pharmaceutical University, Sendai City, Miyagi, Japan.

Methods Mol Biol ; 2613: 127-144, 2023.

Article em En | MEDLINE | ID: mdl-36587076

RESUMO

This chapter describes the protocols for mass spectrometry (MS) applied to the structural characterization of neutral glycosphingolipids (GSLs) and the determination of neutral GSL contents in biological materials. The structural characterization is performed by thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS) and liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) with reversed phase separation. The content determination is carried out by LC-ESI/MS with multiple reaction monitoring (MRM). These protocols provide clues for the functions of neutral GSLs at the level of a single GSL molecular species.

Assuntos

Glicoesfingolipídeos Neutros; Glicoesfingolipídeos Neutros/química; Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos; Espectrometria de Massas por Ionização por Electrospray; Cromatografia Líquida; Cromatografia em Camada Fina/métodos; Glicoesfingolipídeos/química

Palavras-chave

Ceramide structures; Glycan sequences; Glycosphingolipids (GSLs); Liquid chromatography-electrospray ionization mass spectrometry (LC-ESI/MS); Mass spectrometry (MS); Multiple reaction monitoring (MRM); Reversed phase liquid chromatography; Thin layer chromatography-matrix assisted laser desorption ionization/mass spectrometry (TLC-MALDI/MS)

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glicoesfingolipídeos Neutros Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão

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