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Development of a workflow for the detection of vancomycin-resistant Enterococcus faecium and Enterococcus faecalis from rectal swabs using the spectra VRE medium.
Kling, Kendall; Rios, Javier; Dirnberger, Laura; Polanco, Wanda; Fritz, Kevin; Malczynski, Michael; Zembower, Teresa; Qi, Chao.
Afiliação
  • Kling K; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA. kendall.kling@nm.org.
  • Rios J; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Dirnberger L; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Polanco W; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Fritz K; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Malczynski M; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Zembower T; Clinical Microbiology Laboratory, Department of Pathology, Northwestern University Feinberg School of Medicine, 710 N Fairbanks Ct, Chicago, IL, 60611, USA.
  • Qi C; Department of Medicine, Division of Infectious Diseases, Northwestern University Feinberg School of Medicine, 676 N. St. Clair Street Suite 940, Chicago, IL, 60611, USA.
Ann Clin Microbiol Antimicrob ; 22(1): 2, 2023 Jan 06.
Article em En | MEDLINE | ID: mdl-36609280
ABSTRACT

BACKGROUND:

Spectra™ VRE agar (Remel, Lenexa, KS) is a chromogenic agar that is FDA approved for screening patients for VRE colonization. The package insert recommends confirming isolates with identification and susceptibility testing, but confirming every culture delays time to result. Given the agar's historic high specificity for E. faecium isolates, we theorized the agar could be utilized as a stand-alone screening to minimize reagents and time.

AIM:

Our laboratory sought to develop a workflow to optimize the use of the medium.

METHODS:

We plated 3,815 rectal swabs to the Spectra VRE agar and compared results to traditional identification and susceptibility testing.

RESULTS:

Dark blue or purple colonies on the agar demonstrated a sensitivity of 98% and specificity of 85% for detection of VRE faecium, but light blue colonies were significantly less specific for E. faecalis.

CONCLUSIONS:

We streamlined our workflow to accept dark blue or purple colonies as VRE faecium and plan to perform additional testing only on light blue colonies. Interestingly, higher quantity of growth increased the accuracy of the agar. In the future, growth quantity may be used to further streamline the workflow once more data is obtained.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Bactérias Gram-Positivas / Enterococcus faecium / Enterococos Resistentes à Vancomicina Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Ann Clin Microbiol Antimicrob Assunto da revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Bactérias Gram-Positivas / Enterococcus faecium / Enterococos Resistentes à Vancomicina Tipo de estudo: Diagnostic_studies / Prognostic_studies Limite: Humans Idioma: En Revista: Ann Clin Microbiol Antimicrob Assunto da revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos