Cactus-Inspired Photonic Crystal Chip for Attomolar Fluorescence Multi-analysis.

ABSTRACT

Automation and efficiency requirements of environmental monitoring are the pursuit of spontaneous sampling and ultrasensitivity for current sensory systems or detection apparatuses. In this work, inspired by cactus hierarchical structures, we develop a cactus-inspired photonic crystal chip to integrate spontaneous droplet sampling and fluorescence enhancement for sensitive multi-analyte detection. A conical hydrophilic pattern on hydrophobic surfaces can give rise to unidirectional Laplace pressure, which drives droplet transport to the assigned photonic crystal site. The nanostructure of photonic crystals has bigger capillarity to drive the droplet wetting uniformly into the photonic crystal matrix while performing prominent fluorescence enhancement by their photonic bandgap. A low to attomolar (2.24 × 10-19 M) fluorescence limit of detection (LOD) sensitivity can be achieved by the synergy of spontaneous droplet sampling and fluorescence enhancement. Focused on eutrophic water problems and algae pollution monitoring, a femtomolar (1.83 × 10-15 M) LOD and identification of various microcystins in urban environmental water can be achieved. The suitable integration of the unidirectional droplet transport by Laplace pressure and fluorescence enhancement by photonic crystals can achieve the spontaneous sampling and signal enhancement for ultratrace detections and sample survey of environmental monitoring and disease diagnosis.