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A comprehensive survey of C. elegans argonaute proteins reveals organism-wide gene regulatory networks and functions.
Seroussi, Uri; Lugowski, Andrew; Wadi, Lina; Lao, Robert X; Willis, Alexandra R; Zhao, Winnie; Sundby, Adam E; Charlesworth, Amanda G; Reinke, Aaron W; Claycomb, Julie M.
Afiliação
  • Seroussi U; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Lugowski A; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Wadi L; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Lao RX; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Willis AR; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Zhao W; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Sundby AE; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Charlesworth AG; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Reinke AW; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
  • Claycomb JM; Department of Molecular Genetics, University of Toronto, Toronto, Canada.
Elife ; 122023 02 15.
Article em En | MEDLINE | ID: mdl-36790166
ABSTRACT
Argonaute (AGO) proteins associate with small RNAs to direct their effector function on complementary transcripts. The nematode Caenorhabditis elegans contains an expanded family of 19 functional AGO proteins, many of which have not been fully characterized. In this work, we systematically analyzed every C. elegans AGO using CRISPR-Cas9 genome editing to introduce GFP3xFLAG tags. We have characterized the expression patterns of each AGO throughout development, identified small RNA binding complements, and determined the effects of ago loss on small RNA populations and developmental phenotypes. Our analysis indicates stratification of subsets of AGOs into distinct regulatory modules, and integration of our data led us to uncover novel stress-induced fertility and pathogen response phenotypes due to ago loss.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Elife Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Proteínas de Caenorhabditis elegans Limite: Animals Idioma: En Revista: Elife Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá