Analysis of Fat Graft Survival and Platelet-Rich Plasma Effects: The Transcriptomic Differences.
Cureus
; 15(1): e34380, 2023 Jan.
Article
em En
| MEDLINE
| ID: mdl-36874761
ABSTRACT
INTRODUCTION:
Fat graft survival has been studied numerously but has not gone beyond hypothetical solutions. The molecular changes in survival of standard fat grafts and enhanced survival by platelet-rich plasma (PRP) are compared in this study to reveal the etiology that causes the loss of fat grafts after transplantation. MATERIALS ANDMETHODS:
A New Zealand rabbit's inguinal fat pads were excised and divided into three groups Sham, Control (C), and PRP. Each weighing 1 g, C and PRP fat were placed into the bilateral parascapular area of the rabbit. After 30 days, the remaining fat grafts were harvested and weighed (C = 0.7 g, PRP = 0.9 g). All three specimens were put into transcriptome analysis. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes Analysis were done to compare the genetic pathways between the specimens.RESULTS:
Transcriptome analysis showed similar differential expressions in Sham vs. PRP and Sham vs. C comparisons, indicating the dominance of the cellular immune response in both C and PRP specimens. C and PRP comparison resulted in inhibited migration and inflammation pathways in PRP.CONCLUSION:
Fat graft survival is more related to immune responses than any other physiological process. PRP enhances survival by attenuating cellular immune reactions.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
Cureus
Ano de publicação:
2023
Tipo de documento:
Article