The CpxRA two-component system represses gene expression of the heat-labile toxin of enterotoxigenic Escherichia coli.
J Med Microbiol
; 72(4)2023 Apr.
Article
em En
| MEDLINE
| ID: mdl-37043376
ABSTRACT
Enterotoxigenic Escherichia coli (ETEC) strains produce at least one of two types of enterotoxins the heat-labile (LT) and heat-stable (ST) toxins, which are responsible for the watery secretory diarrhoea that is a hallmark of the human ETEC infection. One regulatory system that controls the transcription of virulence genes in pathogenic bacteria is the CpxRA two-component system (TCS). We reported that the eltAB bicistronic operon, which encodes for the A and B subunits of LT, was repressed for the CpxRA TCS by direct binding of CpxR-P from -12 to +6 bp with respect to the transcription start site of eltAB. Moreover, the Cpx-response activation down-regulated the transcription of eltAB genes, and this negative effect was CpxRA-dependent. Our data show that CpxRA TCS is a negative regulator of the LT, one of the main virulence determinants of ETEC.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Toxinas Bacterianas
/
Proteínas de Escherichia coli
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Infecções por Escherichia coli
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Escherichia coli Enterotoxigênica
Limite:
Humans
Idioma:
En
Revista:
J Med Microbiol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
México