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Genetic characterization of dengue virus serotype 1 circulating in Reunion Island, 2019-2021, and the Seychelles, 2015-2016.
Hafsia, Sarah; Barbar, Tatiana; Wilkinson, David A; Atyame, Célestine; Biscornet, Leon; Bibi, Jastin; Louange, Meggy; Gedeon, Jude; De Santis, Olga; Flahault, Antoine; Cabie, André; Bertolotti, Antoine; Mavingui, Patrick.
Afiliação
  • Hafsia S; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, Plateforme CYROI, Sainte Clotilde, La Réunion, France.
  • Barbar T; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, Plateforme CYROI, Sainte Clotilde, La Réunion, France.
  • Wilkinson DA; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, Plateforme CYROI, Sainte Clotilde, La Réunion, France.
  • Atyame C; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, Plateforme CYROI, Sainte Clotilde, La Réunion, France.
  • Biscornet L; Public Health Authority, Ministry of Health, Victoria, Seychelles.
  • Bibi J; Disease Surveillance and Response Unit, Epidemiology and Statistics Section, Public Health Authority, Ministry of Health, Victoria, Seychelles.
  • Louange M; Public Health Authority, Ministry of Health, Victoria, Seychelles.
  • Gedeon J; Public Health Authority, Ministry of Health, Victoria, Seychelles.
  • De Santis O; Institute of global health, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  • Flahault A; Institute of global health, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
  • Cabie A; CHU de Martinique, service de maladies infectieuses et tropicales, INSERM, CHU de Martinique, PCCEI, Univ Montpellier, Univ Antilles, INSERM, EFS, CIC1424, Fort-de-France, Montpellier, France.
  • Bertolotti A; Service des Maladies Infectieuses - Dermatologie, CHU Réunion, INSERM CIC1410, Saint Pierre, Saint Pierre, La Réunion, France.
  • Mavingui P; UMR Processus Infectieux en Milieu Insulaire Tropical (PIMIT), Université de La Réunion, CNRS 9192, INSERM U1187, IRD 249, Plateforme CYROI, Sainte Clotilde, La Réunion, France. patrick.mavingui@cnrs.fr.
BMC Infect Dis ; 23(1): 294, 2023 May 05.
Article em En | MEDLINE | ID: mdl-37147570
BACKGROUND: An unprecedent increase in the number of cases and deaths reported from dengue virus (DENV) infection has occurred in the southwestern Indian ocean in recent years. From 2017 to mid-2021 more than 70,000 confirmed dengue cases were reported in Reunion Island, and 1967 cases were recorded in the Seychelles from 2015 to 2016. Both these outbreaks displayed similar trends, with the initial circulation of DENV-2 which was replaced by DENV-1. Here, we aim to determine the origin of the DENV-1 epidemic strains and to explore their genetic characteristics along the uninterrupted circulation, particularly in Reunion. METHODS: Nucleic acids were extracted from blood samples collected from dengue positive patients; DENV-1 was identified by RT-qPCR. Positive samples were used to infect VERO cells. Genome sequences were obtained from either blood samples or infected-cell supernatants through a combination of both Illumina or MinION technologies. RESULTS: Phylogenetic analyses of partial or whole genome sequences revealed that all DENV-1 sequences from Reunion formed a monophyletic cluster that belonged to genotype I and were closely related to one isolate from Sri Lanka (OL752439.1, 2020). Sequences from the Seychelles belonged to the same major phylogenetic branch of genotype V, but fell into two paraphyletic clusters, with greatest similarity for one cluster to 2016-2017 isolate from Bangladesh, Singapore and China, and for the other cluster to ancestral isolates from Singapore, dating back to 2012. Compared to publicly available DENV-1 genotype I sequences, fifteen non-synonymous mutations were identified in the Reunion strains, including one in the capsid and the others in nonstructural proteins (NS) (three in NS1, two in NS2B, one in NS3, one in NS4B, and seven in NS5). CONCLUSION: In contrast to what was seen in previous outbreaks, recent DENV-1 outbreaks in Reunion and the Seychelles were caused by distinct genotypes, all likely originating from Asia where dengue is (hyper)endemic in many countries. Epidemic DENV-1 strains from Reunion harbored specific non-synonymous mutations whose biological significance needs to be further investigated.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dengue / Vírus da Dengue Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: Africa / Asia Idioma: En Revista: BMC Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dengue / Vírus da Dengue Tipo de estudo: Prognostic_studies Limite: Animals / Humans País/Região como assunto: Africa / Asia Idioma: En Revista: BMC Infect Dis Assunto da revista: DOENCAS TRANSMISSIVEIS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: França