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Identification of ester-linked fatty acids of bacterial endotoxins by negative ion fast atom bombardment mass spectrometry.
Anal Biochem ; 155(1): 168-76, 1986 May 15.
Article em En | MEDLINE | ID: mdl-3717553
ABSTRACT
Negative ion fast atom bombardment mass spectrometry (NI-FAB/MS) was employed to characterize the fatty acids esterified to the lipid A backbone of lipopolysaccharides (LPS) of gram-negative bacteria. LPS and their chemically derived lipid A produced readily detectable fragment ions characteristic of fatty acids. The NI-FAB/MS method is specific, yielding ions indicative of ester- but not of amide-bound fatty acids. The mass spectra of Enterobacteriaceae LPS revealed the presence of lauric (m/z 199), myristic (m/z 227), palmitic (m/z 255), and 3-hydroxymyristic (m/z 243) acids. Pseudomonas aeruginosa LPS gave distinctive fragment ions indicative of 3-hydroxydecanoic (m/z 187), lauric, and 2-hydroxylauric (m/z 215) acids. The Neisseria gonorrhoeae LPS could be distinguished from the others due to the presence of ester-linked 3-hydroxylauric acid. All of the LPS gave abundant ions of m/z 177 and 159, which were derived from diphosphoryl substituents. The use of NI-FAB/MS thus allowed rapid identification of lipid A esterified fatty acids without chemical derivatization or gas chromatographic analysis.
Assuntos
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lipopolissacarídeos / Endotoxinas / Ácidos Graxos Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Anal Biochem Ano de publicação: 1986 Tipo de documento: Article
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Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Lipopolissacarídeos / Endotoxinas / Ácidos Graxos Tipo de estudo: Diagnostic_studies / Prognostic_studies Idioma: En Revista: Anal Biochem Ano de publicação: 1986 Tipo de documento: Article