The effect of extracellular matrix modifications on UDP-glucose dehydrogenase activity in cultured human skin fibroblasts.
Basic Appl Histochem
; 30(1): 85-92, 1986.
Article
em En
| MEDLINE
| ID: mdl-3718422
ABSTRACT
The effect of modifications of the extracellular matrix on the biosynthesis of glycosaminoglycans was investigated in human skin fibroblast cultures by studying UDPGDH activity in order to evaluate a), the histoenzymological and biochemical modifications induced by chondroitinase ABC treatment (new experimental conditions were developed in order to obtain minimum cell damage); b), the reversibility of these modifications; c), the effect of growing the cells in the presence of chondroitinsulfate; d), the specificity of the modifications induced. The results demonstrated that our experimental conditions specifically affected intracellular UDPGDH activity. Chondroitinase ABC treatment induced a reversible increase of UDP-glucuronic acid synthesis. On the contrary, the presence of chondroitinsulfate in the growth medium completely inhibited UDPGDH activity.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Pele
/
Uridina Difosfato Glucose Desidrogenase
/
Desidrogenases de Carboidrato
/
Matriz Extracelular
Limite:
Humans
Idioma:
En
Revista:
Basic Appl Histochem
Ano de publicação:
1986
Tipo de documento:
Article