Hva22, a REEP family protein in fission yeast, promotes reticulophagy in collaboration with a receptor protein.
Autophagy
; 19(10): 2657-2667, 2023 10.
Article
em En
| MEDLINE
| ID: mdl-37191320
ABSTRACT
The endoplasmic reticulum (ER) undergoes selective autophagy called reticulophagy or ER-phagy. Multiple reticulon- and receptor expression enhancing protein (REEP)-like ER-shaping proteins, including budding yeast Atg40, serve as reticulophagy receptors that stabilize the phagophore on the ER by interacting with phagophore-conjugated Atg8. Additionally, they facilitate phagophore engulfment of the ER by remodeling ER morphology. We reveal that Hva22, a REEP family protein in fission yeast, promotes reticulophagy without Atg8-binding capacity. The role of Hva22 in reticulophagy can be replaced by expressing Atg40 independently of its Atg8-binding ability. Conversely, adding an Atg8-binding sequence to Hva22 enables it to substitute for Atg40 in budding yeast. Thus, the phagophore-stabilizing and ER-shaping activities, both of which Atg40 solely contains, are divided between two separate factors, receptors and Hva22, respectively, in fission yeast.Abbreviations AIM:
Atg8-family interacting motif; Atg autophagy related; DTT dithiothreitol; ER endoplasmic reticulum GFP green fluorescent protein; NAA 1-naphthaleneacetic acid; REEP receptor expression enhancing protein; RFP red fluorescent protein; UPR unfolded protein response.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Schizosaccharomyces
/
Autofagia
Idioma:
En
Revista:
Autophagy
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Japão