Adipose delivered stem cells protect liver after ischemia-reperfusion injury by controlling autophagy.
Injury
; 54(8): 110839, 2023 Aug.
Article
em En
| MEDLINE
| ID: mdl-37248113
ABSTRACT
OBJECTIVE:
Ischemia-reperfusion(I/R) injury is an unavoidable side effect of liver surgery and transplantation. A potentially useful tool for cellular therapy and tissue engineering is adipose-derived stem cells (ADSCs).The process of autophagy is used by the cell to break down inappropriate molecules.The study's goal was to examine the impact of ADSCs on the autophagic pathway after rat hepatic ischemia-reperfusion injury. MATERIAL ANDMETHODS:
Thirty male rats used in our study were divided into control, ADSC, ischemia, I/R, and I/R+ ADSC groups (n = 6). Liver tissues were stained with hematoxylin-eosin and histological changes were evaluated with Suzuki scoring. Immunoexpressions of transforming growth factor (TGF-ß) and autophagy markers LC3B, p62 were analyzed using the immunohistochemical method.RESULTS:
As a result of histological evaluation the ischemia and I/R groups displayed sinusoid congestion, vacuolization, and necrosis in liver tissues. We showed that the immunostaining of LC3B and TGF- ß were elevated, and p62 decreased in the rat liver from ischemia and I/R groups when compared to the control group.CONCLUSION:
ADSCs reduced the excessive level of autophagy and structural damage to hepatocytes and the pathological alterations in the liver after ischemia-reperfusion injury.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Traumatismo por Reperfusão
/
Tecido Adiposo
Limite:
Animals
Idioma:
En
Revista:
Injury
Ano de publicação:
2023
Tipo de documento:
Article