[Determination of misoprostol in workplace air by high performance liquid chromatography].
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
; 41(6): 457-460, 2023 Jun 20.
Article
em Zh
| MEDLINE
| ID: mdl-37400409
ABSTRACT
Objective:
To establish a high performance liquid chromatography method for the determination of misoprostol in workplace air.Methods:
From February to August 2021, the misoprostol in the workplace air was collected by glass fiber filter membrane, and theeluent was separated by C18 liquid chromatography column, determined by UV detector, and quantified by external standard method.Results:
The quantitative lower limit of misoprostol determination method was 0.05 µg/ml, and the lowest quantitative concentration was 1.4 µg/m(3) (calculated by collecting 75 L air sample). The concentration of misoprostol has a good linear relationship between 0.05 to 10.00 µg/ml. The relative coefficient was 0.9998. The regression equation of the standard working curve was y=495759x-45257. The range of average recovery rates were from 95.5% to 102.8%. The intra-assay precision of the method was 1.2%-4.6%, and the inter-assay precision was 2.0%-5.9%. The samples could be stored stably for 7 days at 4 â.Conclusion:
The high performance liquid chromatography method for the determination of misoprostol has high sensitivity, good specificity and simple procedure of sample pretreatment. It is suitable for the detection of misoprostol in the workplace air.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Misoprostol
/
Poluentes Ocupacionais do Ar
Idioma:
Zh
Revista:
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi
Assunto da revista:
MEDICINA OCUPACIONAL
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China