High-field magnetic resonance microscopy of aortic plaques in a mouse model of atherosclerosis.
MAGMA
; 36(6): 887-896, 2023 Dec.
Article
em En
| MEDLINE
| ID: mdl-37421501
ABSTRACT
OBJECTIVES:
Pre-clinical models of human atherosclerosis are extensively used; however, traditional histological methods do not allow for a holistic view of vascular lesions. We describe an ex-vivo, high-resolution MRI method that allows the 3 dimensional imaging of the vessel for aortic plaque visualization and quantification. MATERIALS ANDMETHODS:
Aortas from apolipoprotein-E-deficient (apoE-/-) mice fed an atherogenic diet (group 1) or a control diet (group 2) were subjected to 14 T MR imaging using a 3D gradient echo sequence. The obtained data sets were reconstructed (Matlab), segmented, and analyzed (Avizo). The aortas were further sectioned and subjected to traditional histological analysis (Oil-Red O and hematoxylin staining) for comparison.RESULTS:
A resolution up to 15 × 10x10 µm3 revealed that plaque burden (mm3) was significantly (p < 0.05) higher in group 1 (0.41 ± 0.25, n = 4) than in group 2 (0.01 ± 0.01, n = 3). The achieved resolution provided similar detail on the plaque and the vessel wall morphology compared with histology. Digital image segmentation of the aorta's lumen, plaque, and wall offered three-dimensional visualizations of the entire, intact aortas.DISCUSSION:
14 T MR microscopy provided histology-like details of pathologically relevant vascular lesions. This work may provide the path research needs to take to enable plaque characterization in clinical applications.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Aterosclerose
/
Placa Aterosclerótica
Limite:
Animals
/
Humans
Idioma:
En
Revista:
MAGMA
Assunto da revista:
DIAGNOSTICO POR IMAGEM
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos