Partial prevention of compactin (ML-236B) inhibition of in vitro hematopoiesis by dolichol and dolichyl phosphate.
Exp Hematol
; 14(8): 766-70, 1986 Sep.
Article
em En
| MEDLINE
| ID: mdl-3743679
ABSTRACT
We have reported that the exogenous addition of dolichyl phosphate (Dol-P) enhances the colony-forming capacities of early erythroid progenitors (BFU-E), late erythroid progenitors (CFU-E), and granulocyte-macrophage progenitors (CFU-GM) in adult mouse bone marrow, and that dolichol (Dol) enhances that of only CFU-E (Int. J. Cell Cloning 3313, 1985). Compactin (2.5-10 microM), a specific inhibitor of mevalonate biosynthesis that causes a decrease of endogenous Dol biosynthesis, inhibited colony formation of CFU-GM. Exogenous addition of Dol-P partially prevented this inhibition, but Dol and the other mevalonate metabolites, such as cholesterol, coenzyme Q10, and isopentenyladenine, could not. In addition, we have found that the colony-forming capacity of CFU-E in fetal mouse liver was not enhanced by exogenous Dol or Dol-P. But the decrease of colony formation or DNA synthesis of fetal CFU-E in the presence of compactin was prevented by the exogenous addition of Dol or Dol-P.
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Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Lovastatina
/
Diterpenos
/
Dolicóis
/
Hematopoese
/
Naftalenos
Limite:
Animals
Idioma:
En
Revista:
Exp Hematol
Ano de publicação:
1986
Tipo de documento:
Article