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Morpho-cultural, pathological and molecular variability in Phloeospora maculans causing leaf spot of mulberry (Morus species) in India.
Saif, Zakiya; Ashraf, Sabiha; Shah, Mehraj D; Mir, S A; Paddar, B A; Nabi, Asha; Baqual, M F.
Afiliação
  • Saif Z; College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.
  • Ashraf S; College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India. sabiha_ashraf@rediffmail.com.
  • Shah MD; Plant Virology and Molecular Pathology Laboratory, Division of Plant Pathology, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India. mehraj547@rediffmail.com.
  • Mir SA; Plant Virology and Molecular Pathology Laboratory, Division of Plant Pathology, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.
  • Paddar BA; Plant Virology and Molecular Pathology Laboratory, Division of Plant Pathology, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.
  • Nabi A; Plant Virology and Molecular Pathology Laboratory, Division of Plant Pathology, Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.
  • Baqual MF; College of Temperate Sericulture, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, Srinagar, Jammu, 190025, Kashmir, India.
Mol Biol Rep ; 50(10): 8337-8348, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37592179
BACKGROUND: Leaf spot disease (LSD) of mulberry caused by Phloeospora maculans is a major threat to the silk industry of Jammu and Kashmir, India, therefore, it was necessary to study the population structure of the pathogen for successful management of the disease. METHODS AND RESULTS: To understand the diversity in the Phloeospora maculans, a combination of conventional (morphological, cultural and pathological) and molecular (ISSR markers) approaches were employed to discern the variability in 27 isolates collected from Srinagar, Bandipora, and Baramulla districts of Jammu and Kashmir, India. The studies revealed a high level of variability in the pathogen. Based on the morpho-cultural and pathological studies, the pathogen isolates were grouped into different categories based on colony growth, texture, margin and colour besides changes in colour of medium, incubation period, leaf area infected, etc.A high level of polymorphism was observed in different isolates of P. maculans using ISSR markers, which indicated that these markers are suitable for studying the genetic diversity in this pathogen. All the isolates (27) of P. maculans were clustered into two groups or populations as indicated by mean delta K value. Analysis of molecular variance revealed the low genetic variation among the populations (1.08%) and a high level of genetic variation within the populations (98.91%). Fst value was found to be 0.01 indicating smaller amount of genetic differentiation between the populations against calculated P-value of 0.29. CONCLUSION: A high level of diversity based on morphological, cultural, pathological and molecular levels was observed in Phloeospora maculans collected from various districts of Kashmir valley, which indicates that the study of population structure is necessary for successful management of the disease.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Morus País/Região como assunto: Asia Idioma: En Revista: Mol Biol Rep Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ascomicetos / Morus País/Região como assunto: Asia Idioma: En Revista: Mol Biol Rep Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia