Saccharomyces cerevisiae NRE1 and IRC24 Encode Paralogous Benzil Oxidoreductases.
MicroPubl Biol
; 20232023.
Article
em En
| MEDLINE
| ID: mdl-37602278
ABSTRACT
Irc24p is a benzil oxidoreductase encoded on chromosome IX of Saccharomyces cerevisiae . We identified a putative paralog, Nre1p, encoded 284 bp downstream. Both proteins are small, cytoplasmic, and are 52% identical (70% similar). PANTHER and PFAM analysis of the amino acid sequences and rigid pairwise structure alignment predicted a conserved active site and Rossmann folds in both, implicating NADH or NADPH as likely cofactors. We purified hexahistidine-tagged Irc24p and Nre1p. Both proteins catalyze the reduction of the diketone benzil with similar kinetics and a preference for NADPH. This is the first demonstration of in vitro function for Nre1p.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Idioma:
En
Revista:
MicroPubl Biol
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Estados Unidos