Direct screening for effective shRNA with a single mismatch in human cells without laborious cloning.

Tachibana, Mayu; Ehara, Nazumi; Tanikawa, Shunya; Tachibana, Akira

Tachibana, Mayu; Ehara, Nazumi; Tanikawa, Shunya; Tachibana, Akira.

Afiliação

Tachibana M; Department of Chemistry and Bioengineering, Division of Science and Engineering for Materials, Chemistry and Biology, Graduate School of Engineering, Osaka Metropolitan University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Ehara N; Department of Chemistry and Bioengineering, Division of Science and Engineering for Materials, Chemistry and Biology, Graduate School of Engineering, Osaka Metropolitan University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Tanikawa S; Department of Chemistry and Bioengineering, Division of Science and Engineering for Materials, Chemistry and Biology, Graduate School of Engineering, Osaka Metropolitan University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.
Tachibana A; Department of Chemistry and Bioengineering, Division of Science and Engineering for Materials, Chemistry and Biology, Graduate School of Engineering, Osaka Metropolitan University, Sugimoto 3-3-138, Sumiyoshi-ku, Osaka 558-8585, Japan.

Biosci Biotechnol Biochem ; 88(1): 70-73, 2023 Dec 19.

Article em En | MEDLINE | ID: mdl-37793878

ABSTRACT

ABSTRACT

We have devised a method for the direct screening of efficient short hairpin (sh)RNA molecules in human cells, eliminating the need for the time-consuming process of cloning in Escherichia coli. Our screening suggested that single mismatches to shRNAs can significantly alter their activity.

Assuntos

Vetores Genéticos; Humanos; RNA Interferente Pequeno/genética; Clonagem Molecular; Interferência de RNA

Palavras-chave

direct screening in human cells; prior to plasmid construction; shRNA; single mismatch

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vetores Genéticos Tipo de estudo: Diagnostic_studies / Screening_studies Limite: Humans Idioma: En Revista: Biosci Biotechnol Biochem Assunto da revista: BIOQUIMICA / BIOTECNOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Japão

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