An optimized protocol for the generation and monitoring of conditional orthotopic lung cancer in the KP mouse model using an adeno-associated virus vector compatible with biosafety level 1.

Deng, Haibin; Ge, Huixiang; Dubey, Christelle; Losmanova, Tereza; Medová, Michaela; Konstantinidou, Georgia; Mutlu, Seyran Mathilde; Birrer, Fabienne Esther; Brodie, Tess Melinda; Stroka, Deborah; Wang, Wenxiang; Peng, Ren-Wang; Dorn, Patrick; Marti, Thomas Michael

Deng, Haibin; Ge, Huixiang; Dubey, Christelle; Losmanova, Tereza; Medová, Michaela; Konstantinidou, Georgia; Mutlu, Seyran Mathilde; Birrer, Fabienne Esther; Brodie, Tess Melinda; Stroka, Deborah; Wang, Wenxiang; Peng, Ren-Wang; Dorn, Patrick; Marti, Thomas Michael.

Afiliação

Deng H; Department of General Thoracic Surgery, Inselspital, Bern University Hospital, University of Bern, Murtenstrasse 28, 3008, Bern, Switzerland.
Ge H; Department for BioMedical Research, University of Bern, Bern, Switzerland.
Dubey C; Thoracic Surgery Department 2, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, Hunan, China.
Losmanova T; Hunan Key Laboratory of Translational Radiation Oncology, Hunan Cancer Hospital and the Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha, 410013, China.
Medová M; Department of General Thoracic Surgery, Inselspital, Bern University Hospital, University of Bern, Murtenstrasse 28, 3008, Bern, Switzerland.
Konstantinidou G; Department for BioMedical Research, University of Bern, Bern, Switzerland.
Mutlu SM; Graduate School of Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland.
Birrer FE; Department of General Thoracic Surgery, Inselspital, Bern University Hospital, University of Bern, Murtenstrasse 28, 3008, Bern, Switzerland.
Brodie TM; Department for BioMedical Research, University of Bern, Bern, Switzerland.
Stroka D; Institute of Pathology, University of Bern, Bern, Switzerland.
Wang W; Department for BioMedical Research, University of Bern, Bern, Switzerland.
Peng RW; Department of Radiation Oncology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Dorn P; Institute of Pharmacology, University of Bern, Bern, Switzerland.
Marti TM; Department for BioMedical Research, University of Bern, Bern, Switzerland.

Cancer Immunol Immunother ; 72(12): 4457-4470, 2023 Dec.

Article em En | MEDLINE | ID: mdl-37796299

Assuntos

Adenocarcinoma de Pulmão; Neoplasias Pulmonares; Camundongos; Animais; Humanos; Neoplasias Pulmonares/genética; Neoplasias Pulmonares/patologia; Proteínas Proto-Oncogênicas p21(ras)/genética; Dependovirus/genética; Microtomografia por Raio-X; Proteína Supressora de Tumor p53; Contenção de Riscos Biológicos; Modelos Animais de Doenças; Vetores Genéticos/genética

Palavras-chave

AAV vector; BSL-1; Cre-expressing vector; Intratracheal instillation; Lung adenocarcinoma; Mouse model

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Adenocarcinoma de Pulmão / Neoplasias Pulmonares Tipo de estudo: Guideline / Risk_factors_studies Limite: Animals / Humans Idioma: En Revista: Cancer Immunol Immunother Assunto da revista: ALERGIA E IMUNOLOGIA / NEOPLASIAS / TERAPEUTICA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Suíça

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