Detection of wildtype Merkel cell polyomavirus genomic sequence and VP1 transcription in a subset of Merkel cell carcinoma.
Histopathology
; 84(2): 356-368, 2024 Jan.
Article
em En
| MEDLINE
| ID: mdl-37830288
ABSTRACT
AIMS:
Merkel cell carcinoma (MCC) is frequently caused by the Merkel cell polyomavirus (MCPyV). Characteristic for these virus-positive (VP) MCC is MCPyV integration into the host genome and truncation of the viral oncogene Large T antigen (LT), with full-length LT expression considered as incompatible with MCC growth. Genetic analysis of a VP-MCC/trichoblastoma combined tumour demonstrated that virus-driven MCC can arise from an epithelial cell. Here we describe two further cases of VP-MCC combined with an adnexal tumour, i.e. one trichoblastoma and one poroma. METHODS ANDRESULTS:
Whole-genome sequencing of MCC/trichoblastoma again provided evidence of a trichoblastoma-derived MCC. Although an MCC-typical LT-truncating mutation was detected, we could not determine an integration site and we additionally detected a wildtype sequence encoding full-length LT. Similarly, Sanger sequencing of the combined MCC/poroma revealed coding sequences for both truncated and full-length LT. Moreover, in situ RNA hybridization demonstrated expression of a late region mRNA encoding the viral capsid protein VP1 in both combined as well as in a few cases of pure MCC.CONCLUSION:
The data presented here suggest the presence of wildtype MCPyV genomes and VP1 transcription in a subset of MCC.Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Cutâneas
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Neoplasias das Glândulas Sudoríparas
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Carcinoma de Célula de Merkel
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Infecções por Polyomavirus
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Poroma
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Poliomavírus das Células de Merkel
Limite:
Humans
Idioma:
En
Revista:
Histopathology
Ano de publicação:
2024
Tipo de documento:
Article
País de afiliação:
França