Emergence of OXA-48-producing Enterobacter hormaechei in a Swiss companion animal clinic and their genetic relationship to clinical human isolates.
J Antimicrob Chemother
; 78(12): 2950-2960, 2023 12 01.
Article
em En
| MEDLINE
| ID: mdl-37923369
ABSTRACT
BACKGROUND:
Enterobacter hormaechei producing the carbapenemase OXA-48 was identified repeatedly in infections in companion animals hospitalized at a Swiss veterinary clinic where OXA-48-producing Klebsiella pneumoniae was previously reported.OBJECTIVES:
To determine the genetic relatedness of animal and human E. hormaechei strains collected in Switzerland during 2017-22 and their mobile genetic elements.METHODS:
Hybrid assemblies for phylogenetic and comparative analysis of animal (nâ=â9) and human (nâ=â25) isolates were obtained by sequencing with Illumina, PacBio and Oxford Nanopore Technologies. Antimicrobial susceptibility was tested by broth microdilution.RESULTS:
The animal strains were identified as E. hormaechei subsp. xiangfangensis ST114 (nâ=â6) and ST418 (nâ=â2), and E. hormaechei subsp. hoffmannii ST78 (nâ=â1). Human E. hormaechei belonged to subspecies steigerwaltii (nâ=â10), xiangfangensis (nâ=â13), hoffmannii (nâ=â1) and hormaechei (nâ=â1), with a heterogeneous ST distribution differing from the animal strains, except for two ST114. Core-gene SNP analysis confirmed the clonality of the animal ST114 and ST418 isolates (0 to 10 SNPs), and close relatedness of animal and human ST114 strains (80-120 SNPs). The strains harboured the blaOXA-48 gene on ca. 63â kb IncL-type plasmids (nâ=â27); on ca. 72â kb IncL plasmids co-harbouring blaCTX-M-14 (nâ=â2); and on ca. 150-180â kb IncFIB (nâ=â4) or hybrid IncFIB/IncL (nâ=â1) plasmids. The blaOXA-48-harbouring plasmids and the blaDHA-1-carrying ISCR1 element in one animal ST114 and both ST418 clones were likely acquired from previously spreading K. pneumoniae strains.CONCLUSIONS:
Common ecological niches favour the spread of plasmid-borne carbapenemases among Enterobacterales and the emergence of MDR E. hormaechei clones.
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Infecções por Klebsiella
/
Animais de Estimação
Limite:
Animals
/
Humans
País/Região como assunto:
Europa
Idioma:
En
Revista:
J Antimicrob Chemother
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
Suíça