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Protocol for isolating mice skeletal muscle myoblasts and myotubes via differential antibody validation.
Stephens, Dominique C; Mungai, Margaret; Crabtree, Amber; Beasley, Heather K; Garza-Lopez, Edgar; Vang, Larry; Neikirk, Kit; Vue, Zer; Vue, Neng; Marshall, Andrea G; Turner, Kyrin; Shao, Jian-Qiang; Sarker, Bishnu; Murray, Sandra; Gaddy, Jennifer A; Davis, Jamaine; Damo, Steven M; Hinton, Antentor O.
Afiliação
  • Stephens DC; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA; Department of Life and Physical Sciences, Fisk University, Nashville, TN 37232, USA.
  • Mungai M; Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.
  • Crabtree A; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Beasley HK; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Garza-Lopez E; Department of Internal Medicine, University of Iowa, Iowa City, IA 52242, USA.
  • Vang L; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Neikirk K; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Vue Z; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Vue N; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Marshall AG; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA.
  • Turner K; Department of Life and Physical Sciences, Fisk University, Nashville, TN 37232, USA.
  • Shao JQ; Central Microscopy Research Facility, University of Iowa, Iowa City, IA 52242, USA.
  • Sarker B; School of Applied Computational Sciences, Meharry Medical College, Nashville, TN 37232, USA.
  • Murray S; Department of Cell Biology, College of Medicine, University of Pittsburgh, Pittsburgh, TN 15260, USA.
  • Gaddy JA; Division of Infectious Diseases, Vanderbilt University School of Medicine, Nashville, TN, USA; Tennessee Valley Healthcare Systems, U.S. Department of Veterans Affairs, Nashville, TN, USA.
  • Davis J; Department of Biochemistry and Cancer Biology. Meharry Medical College, Nashville, TN, USA.
  • Damo SM; Department of Life and Physical Sciences, Fisk University, Nashville, TN 37232, USA. Electronic address: sdamo@fisk.edu.
  • Hinton AO; Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN 37232, USA. Electronic address: antentor.o.hinton.jr@vanderbilt.edu.
STAR Protoc ; 4(4): 102591, 2023 Dec 15.
Article em En | MEDLINE | ID: mdl-37938976
Isolation of skeletal muscles allows for the exploration of many complex diseases. Here, we present a protocol for isolating mice skeletal muscle myoblasts and myotubes that have been differentiated through antibody validation. We describe steps for collecting and preparing murine skeletal tissue, myoblast cell maintenance, plating, and cell differentiation. We then detail procedures for cell incubation, immunostaining, slide preparation and storage, and imaging for immunofluorescence validation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Músculo Esquelético / Fibras Musculares Esqueléticas Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Músculo Esquelético / Fibras Musculares Esqueléticas Limite: Animals Idioma: En Revista: STAR Protoc Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Estados Unidos