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Immersion prime and oral boost vaccination with an inactivated Vibrio harveyi vaccine confers a specific immune response and protection in Asian seabass (Lates calcarifer).
Lan, Nguyen Giang Thu; Dong, Ha Thanh; Vinh, Nguyen Tien; Senapin, Saengchan; Shinn, Andrew P; Salin, Krishna R; Rodkhum, Channarong.
Afiliação
  • Lan NGT; The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Fish Infectious Diseases (CE FID), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn Un
  • Dong HT; Aquaculture and Aquatic Resources Management, Department of Food Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Pathum Thani, Thailand. Electronic address: htdong@ait.ac.th.
  • Vinh NT; Aquaculture and Aquatic Resources Management, Department of Food Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Pathum Thani, Thailand.
  • Senapin S; National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, Thailand; Fish Heath Platform, Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp), Faculty of Science, Mahidol University, Ba
  • Shinn AP; INVE (Thailand), 471 Bond Street, Bangpood, Pakkred, Nonthaburi, 11120, Thailand.
  • Salin KR; Aquaculture and Aquatic Resources Management, Department of Food Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, Pathum Thani, Thailand.
  • Rodkhum C; The International Graduate Program of Veterinary Science and Technology (VST), Faculty of Veterinary Science, Chulalongkorn University, Bangkok, Thailand; Center of Excellence in Fish Infectious Diseases (CE FID), Department of Veterinary Microbiology, Faculty of Veterinary Science, Chulalongkorn Un
Fish Shellfish Immunol ; 144: 109293, 2024 Jan.
Article em En | MEDLINE | ID: mdl-38104696
ABSTRACT
Asian seabass (Lates calcarifer) holds significant economic value in fish farming in the Asia-Pacific region. Vibriosis caused by Vibrio harveyi (Vh) is a severe infectious disease affecting intensive farming of this species, for which prevention strategies by vaccination have been developed. This study investigated an alternative approach to injectable vaccination to prevent vibriosis in Asian seabass juveniles. The strategy begins with an immersion prime vaccination with a heat-inactivated Vh vaccine, followed by two oral booster doses administered at 14- and 28-days post-vaccination (dpv). Expression of five immune genes TNFα, IL1ß, CD4, CD8, and IgM in the head kidney and spleen, along with investigation of anti-Vh antibody response (IgM) in both systemic and mucosal systems, was conducted on a weekly basis. The efficacy of the vaccines was assessed by a laboratory challenge test at 43 dpv. The results showed that the immunized fish displayed higher levels of mRNA transcripts of the immune genes after the immersion prime and the first oral booster dose compared to the control group. The expression levels peaked at 14 and 28 dpv and then declined to baseline at 35 and 42 dpv. Serum specific IgM antibodies were detected as early as 7 dpv (the first time point investigated) and exhibited a steady increase, reaching the first peak at 21 dpv, and a second peak at 35 dpv. Although the antibody levels gradually declined over subsequent weeks, they remained significantly higher than the control group throughout the experiment. A similar antibody response pattern was also observed in the mucosal compartment. The laboratory challenge test demonstrated high protection by injection with 1.65 × 104 CFU/fish, with a relative percent of survival (RPS) of 72.22 ± 7.86 %. In conclusion, our findings highlight the potential of an immersion prime-oral booster vaccination strategy as a promising approach for preventing vibriosis in Asian seabass.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrioses / Bass / Perciformes / Vacinas Bacterianas / Doenças dos Peixes Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Vibrioses / Bass / Perciformes / Vacinas Bacterianas / Doenças dos Peixes Limite: Animals Idioma: En Revista: Fish Shellfish Immunol Assunto da revista: BIOLOGIA / MEDICINA VETERINARIA Ano de publicação: 2024 Tipo de documento: Article